cgtr - Discovery of Genetic Signatures and Bioactive Molecules

qNPA ASSAY

Per HTG, "The quantitative nuclease protection assay (qNPA) allows researchers to quickly and accurately measure the gene expression levels in a wide range of mammalian, plant and bacterial cell types. The fast, automated protocol requires no RNA extraction, no RNA amplification, and no RNA labeling, leading to more reproducible results." For more information on qNPA technology, visit HTG.

Related Publications

Roberts R.A., Sabalos C.M., Leblanc M.L., Martel R.R., Frutiger Y.M., Unger J.M., Botros I.W., Rounseville M.P., Seligmann B.E., Miller T.P., Grogan T.M., Rimsza L.M. (2007) “Quantitative nuclease protection assay in paraffin-embedded tissue replicates prognostic microarray gene expression in diffuse large-B-cell lymphoma”, Lab Invest. 87(10), 979-997.

Kris, R.M., Felder, S., Deyholos, M., Lambert, G.M., Hinton, J., Botros,I., Martel, R., Seligmann, B., Galbraith D.W. (2007) “High-Throughput, High-Sensitivity Analysis of Gene Expression in Arabidopsis”, Plant Physiology, 144, 1256-1266.

Sawada H., Taniguchi K., Takami K. (2006) “Improved toxicogenomic screening for drug-induced phospholipidosis using a multiplexed quantitative gene expression ArrayPlate assay”, Toxicol In Vitro. 20(8):1506-13

Martel, R.R., Rounseville, M.P., Botros, I.W. and Seligmann, B.E. (2004) “Array Formats” pp. 3-22 in “Microarray Technology and Its Applications”, ISBN: 3-540-22931-0, Müller, U.R. and Nicolau, D.V., Eds., Springer-Verlag, Heidelberg.

Martel, R. (2003) “Arrays of Possibilities”, Modern Drug Discovery (Published by The American Chemical Society), November 2003, 47-50.

Palfreyman M.G., Hook D.J., Klimczak L.J., Brockman J.A., Evans D.M., Altar C.A. (2002) “Novel directions in antipsychotic target identification using gene arrays” Curr Drug Targets CNS Neurol Disord. 1(2):227-38.

Martel, R.R., Botros, I.W., Rounseville, M.P., Hinton, J.P., Staples, R.R., Morales, D.A., Farmer, J.B., and Seligmann, B.E. (2002) “Multiplexed Screening Assay for mRNA Combining Nuclease Protection with Luminescent Array Detection”, Assay and Drug Development Technologies, 1(1), 61-71.

Martel, R.R., Rounseville, M.P., Botros, I.W. and Seligmann, B.E. (2002) “Multiplexed Chemiluminescent Assays in ArrayPlates for High-Throughput Measurement of Gene Expression”, Progress in Biomedical Optics and Imaging, 3(18), 35-43.

Martel, R.R., Rounseville, M.P., Botros, I.W., Kris, R., Felder, S. and Seligmann, B.E. (2002) “Multiplexed Molecular Profiling (MMP) Transcription Assay in ArrayPlates for High-Throughput Measurement of Gene Expression” pp. 549-564 in “Gene Cloning and Expression Technologies”, ISBN: 1-881299-20-1, Lu, Q. and Weiner, M., Eds., BioTechniques Press, Eaton Publishing, Westborough, MA.